Table 1

Composition of experimental diets

Ingredient

Low-Fe Bean Diet

High-Fe Bean Diet


g/kg (by formulation)


High-Fe Beans (71 μg Fe/g)

_

600


Low-Fe Beans (49 μg Fe/g)

600

_


Corn

200

200


Corn oil

30

30


Dry skim milk

100

100


Vitamin/mineral premix (no Fe) 1

70

70


DL-Methionine

2.5

2.5


Choline Chloride

0.75

0.75


Total (g)

1000

1000


Concentrations of selected components

mean ± SEM, n = 5 (by analysis)4


Fe (μg Fe/g) 2

42.9 ± 1.2a

54.6 ± 0.9b


Total Phenols (gallic acid, μg/g) 3

103.5 ± 5.5a

101.8 ± 6.1a


Phytate:Fe molar ratio 3

8.28 ± 9.2a

8.59 ± 1.06a


1Vitamin and mineral premix provided/kg diet (330002 Chick vitamin mixture; 230000 Salt mix for chick diet; Dyets Inc. Bethlehem, PA).

2Iron concentrations in the diets were determined by an inductively-coupled argon-plasma/atomic emission spectrophotometer (ICAP 61E Thermal Jarrell Ash Trace Analyzer, Jarrell Ash Co. Franklin, MA) following wet ashing.

3Method for determining phenol concentrations and phytate contents are described in the materials and methods section.

4Within a row, means without a common letter are significantly different, P < 0.05.

Tako et al. Nutrition Journal 2011 10:113   doi:10.1186/1475-2891-10-113

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