Table 1 |
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Composition of experimental diets |
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Ingredient |
Low-Fe Bean Diet |
High-Fe Bean Diet |
|
|
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|
g/kg (by formulation) |
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|
|
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|
High-Fe Beans (71 μg Fe/g) |
_ |
600 |
|
|
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|
Low-Fe Beans (49 μg Fe/g) |
600 |
_ |
|
|
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|
Corn |
200 |
200 |
|
|
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|
Corn oil |
30 |
30 |
|
|
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|
Dry skim milk |
100 |
100 |
|
|
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|
Vitamin/mineral premix (no Fe) 1 |
70 |
70 |
|
|
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|
DL-Methionine |
2.5 |
2.5 |
|
|
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|
Choline Chloride |
0.75 |
0.75 |
|
|
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|
Total (g) |
1000 |
1000 |
|
|
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|
Concentrations of selected components |
mean ± SEM, n = 5 (by analysis)4 |
|
|
|
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|
Fe (μg Fe/g) 2 |
42.9 ± 1.2a |
54.6 ± 0.9b |
|
|
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|
Total Phenols (gallic acid, μg/g) 3 |
103.5 ± 5.5a |
101.8 ± 6.1a |
|
|
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|
Phytate:Fe molar ratio 3 |
8.28 ± 9.2a |
8.59 ± 1.06a |
|
|
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|
1Vitamin and mineral premix provided/kg diet (330002 Chick vitamin mixture; 230000 Salt mix for chick diet; Dyets Inc. Bethlehem, PA). 2Iron concentrations in the diets were determined by an inductively-coupled argon-plasma/atomic emission spectrophotometer (ICAP 61E Thermal Jarrell Ash Trace Analyzer, Jarrell Ash Co. Franklin, MA) following wet ashing. 3Method for determining phenol concentrations and phytate contents are described in the materials and methods section. 4Within a row, means without a common letter are significantly different, P < 0.05. |
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Tako et al. Nutrition Journal 2011 10:113 doi:10.1186/1475-2891-10-113 |
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