Table 1 |
||||||||
|
Summary of assay validation results. |
||||||||
|
Compounds |
Linear range (ng/mL) |
LLOD (ng/mL) |
Recovery (%) at 50 ng/mL |
Concentration (ng/mL) |
r2 |
Intraday (%CV) |
Interday (%CV) |
Accuracy (%) |
|
|
||||||||
|
25OHD3 |
0.5-84.4 |
0.25 |
95 |
12.5 |
0.9999 |
2.1 |
12.1 |
97.5 |
|
25 |
4.6 |
6.7 |
92.9 |
|||||
|
50 |
7.1 |
4.1 |
92.2 |
|||||
|
25OHD2 |
0.5-84.4 |
0.25 |
88 |
12.5 |
0.9996 |
7.5 |
8.1 |
99.4 |
|
25 |
9.1 |
8.6 |
97.5 |
|||||
|
50 |
6.8 |
5.5 |
101.2 |
|||||
|
|
||||||||
|
Shah et al. Nutrition Journal 2011 10:46 doi:10.1186/1475-2891-10-46 |
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