Table 1

Summary of assay validation results.

Compounds

Linear range (ng/mL)

LLOD (ng/mL)

Recovery (%) at 50 ng/mL

Concentration (ng/mL)

r2

Intraday (%CV)

Interday (%CV)

Accuracy (%)


25OHD3

0.5-84.4

0.25

95

12.5

0.9999

2.1

12.1

97.5

25

4.6

6.7

92.9

50

7.1

4.1

92.2

25OHD2

0.5-84.4

0.25

88

12.5

0.9996

7.5

8.1

99.4

25

9.1

8.6

97.5

50

6.8

5.5

101.2


Shah et al. Nutrition Journal 2011 10:46   doi:10.1186/1475-2891-10-46

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