Table 2

Retention times, MRM transitions and ESI conditions of analytes.

Analytes

Retention time (min)

Transition (m/z) Precursor→ Product

Collision energy (eV)

401.3 → 383.1

36

25OHD3

13.5

401.3 → 365.1

41

401.3 → 159.2

46

413.3 → 395.5

46

25OHD2

11.1

413.3 → 377.2

51

413.3 → 355.5

53

401.3 → 383.1

36

3-epi-25OHD3

13.1

401.3 → 365.1

41

401.3 → 159.2

46

401.3 → 383.1

36

1αOHD3

11.1

401.3 → 365.1

41

401.3 → 159.2

46

401.3 → 383.1

36

7αC4

10.5

401.3 → 365.1

41

401.3 → 159.2

46

Stanozolol-D3 (I.S)

3.09

332.2 → 81.2

42

Shah et al. Nutrition Journal 2011 10:46   doi:10.1186/1475-2891-10-46