Figure 1.

Effect of mastic gum on cellular production of O₂* and H₂O₂. (A) Production of intracellular O₂* was measured by DHE following accumulation of 2-hydroxyethidium using HPLC as described in Materials and Methods [21]. RASMC were stimulated with 20 ng/ml TNF-α for 4-hours. Cells were supplemented with various doses of mastic gum (0-10 μg/ml) for 15-minutes prior to measurements of superoxide. (B) Production of cellular H₂O₂ was measured by Amplex Red as described in Materials and Methods [21]. RASMC were stimulated with 20 ng/ml TNF-α for 4-hours and then supplemented with various doses of mastic gum (0-10 μg/ml) for 15-minutes prior to measurements of H₂O₂. *P < 0.01 vs Control, **P < 0.01 vs TNF-α. (C) Superoxide production in bovine aortic endothelial cells (BAEC) stimulated with 200 nM angiotensin II (Ang II) for 4-hours and treated with mastic gum. Control unstimulated BAEC or Ang II-stimulated BAEC were supplemented with 10 μg/ml mastic gum for 15-minutes prior to measurements of superoxide. Data are average from six to eight separate experiments ± Standard Error. *P < 0.01 vs Control, **P < 0.01 vs Ang II.