Open Access Open Badges Research

Acute reduction of serum 8-iso-PGF2-alpha and advanced oxidation protein products in vivo by a polyphenol-rich beverage; a pilot clinical study with phytochemical and in vitro antioxidant characterization

Boris V Nemzer1*, Liliana C Rodriguez2, Linda Hammond2, Robert DiSilvestro3, John M Hunter1 and Zbigniew Pietrzkowski4

Author Affiliations

1 FutureCeuticals, Inc. 2692 N. State Rt. 1-17, Momence, IL 60954, USA

2 NutraClinical, Inc., 5755 Oberlin Drive, Suite 301, San Diego, CA 92121, USA

3 Human Nutrition, Ohio State University, Columbus, OH 43210, USA

4 Applied BioClinical, Inc. 16259 Laguna Canyon Road, Suite 150, Irvine, CA 92618, USA

For all author emails, please log on.

Nutrition Journal 2011, 10:67  doi:10.1186/1475-2891-10-67

Published: 15 June 2011



Measuring the effects of the acute intake of natural products on human biomarker concentrations, such as those related to oxidation and inflammation, can be an advantageous strategy for early clinical research on an ingredient or product.


31 total healthy subjects were randomized in a double-blinded, placebo-controlled, acute pilot study with post-hoc subgroup analysis on 20 of the subjects. The study examined the effects of a single dose of a polyphenol-rich beverage (PRB), commercially marketed as "SoZo®", on serum anti-inflammatory and antioxidant markers. In addition, phytochemical analyses of PRB, and in vitro antioxidant capacity were also performed.


At 1 hour post-intake, serum values for 8-iso-PGF2-alpha and advanced oxidation protein products decreased significantly by 40% and 39%, respectively. Additionally, there was a trend toward decreased C-reactive protein, and increased nitric oxide levels. Both placebo and PRB treatment resulted in statistically significant increases in hydroxyl radical antioxidant capacity (HORAC) compared to baseline; PRB showed a higher percent change (55-75% versus 23-74% in placebo group), but the two groups did not differ significantly from each other.


PRB produced statistically significant changes in several blood biomarkers related to antioxidant/anti-inflammatory effects. Future studies are justified to verify results and test for cumulative effects of repeated intakes of PRB. The study demonstrates the potential utility of acute biomarker measurements for evaluating antioxidant/anti-inflammatory effects of natural products.

polyphenols; beverage; 8-iso-PGF2-alpha; AOPP; NO; antioxidant