Figure 2.

(A) Effect of vitamin A supplementation on hepatic 11β-HSD1 activity in WNIN/Ob lean and obese rats. Values are means ± S.E for 8 rats. Mean values with * mark are significant at P ≤0.05 level (by one-way ANOVA). (B) Effect of vitamin A supplementation on 11β-HSD1 gene expression in liver of WNIN/Ob lean and obese rats. Quantified densitometric values are expressed relative of 1 for lean control rats (AI). (C) Representative photo of 11β-HSD1 PCR product, stained by ethidium bromoide. (D) Representative photo of PCR β-Actin product (internal control), stained by ethidium bromide. Comparisons were made between normal and high vitamin A-fed groups of each phenotype (AI-Lean: 2.6 mg vitamin A/kg diet, AII-Lean: 129 mg vitamin A/kg diet, BI-Obese: 2.6 mg vitamin A/kg diet and BII-Obese: 129 mg/kg diet).