Microscopy characterization of the in vitro intestinal epithelium model. Cross-sectional images from Caco-2 grown on Transwell filters to form confluent monolayers of differentiated cell are in panels A and B. A junction -rich, organized field is in panel A (JAM white, DAPI blue). Another field with well-developed apical brush border (DPPIV red) is in panel B. Inset in the bottom right corner of panel B displays the 2D free projection max of the same. In panel C Hematoxylin and Eosin stain shows a transversal cryosection from a filter with cells treated with MnOxP for 30 min. The morphology, i.e. columnar cells forming a tight and regular monolayer, appears preserved.
Foglieni et al. Nutrition Journal 2011 10:77 doi:10.1186/1475-2891-10-77