Confocal microscopy of polarized Caco-2 cells treated with MnGluc or MnOxP- analysis of MT fluorescence. Quantification and distribution of MT in response to 12.5 μM MnGluc or MnOxP are shown. Root Mean Square fluorescence intensity quantification from a representative long experiment is in panel A. Symbols (o, #, *, §) indicate significant differences (p < 0.05) between Caco-2 cells responses. Panel B shows confocal MT images from the same samples at 30 min and 24 h, in agreement with data in panel A and displaying that the regular mitochondria distribution is preserved in treated cells. Transversal 2D free projection max (panel C) from a brief experiment displays both AO and MT signals in the presence of MnOxP. MT shows a transient redistribution of mitochondria close to the apex of cells at 15 min. AO helps in localize the nuclei respect to MT and demonstrates the cells viability.
Foglieni et al. Nutrition Journal 2011 10:77 doi:10.1186/1475-2891-10-77