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Bioactive properties and clinical safety of a novel milk protein peptide

Richard B Kreider1*, Mike Iosia2, Matt Cooke3, Geoffrey Hudson4, Chris Rasmussen1, Helen Chen5, Olof Mollstedt5 and Men-Hwei Tsai5

Author Affiliations

1 Exercise and Sport Nutrition Laboratory, Department of Health & Kinesiology, Texas A&M University College Station, TX 77843-4243, USA

2 Department of Health, Exercise Science, and Secondary Education, Lee University, Cleveland, TN 37320, USA

3 Schools of Biomedical & Health Sciences, Victoria University, Melbourne Victoria 8001, Australia

4 School of Human Performance and Recreation, University of Southern Mississippi, Hattiesburg, MS 39406, USA

5 Ambryx Biotechnology, Inc., Riverside, CA, 92507-2155, USA

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Nutrition Journal 2011, 10:99  doi:10.1186/1475-2891-10-99

Published: 26 September 2011



Milk protein fractions and peptides have been shown to have bioactive properties. This preliminary study examined the potential mechanisms of action and clinical safety of novel milk protein peptide (MP).


A novel MP mixture inhibits the tyrosine kinase activity of epidermal growth factor receptor (EGFR), vascular endothelial growth factor receptor 2 (VEGFR2), and insulin receptor (IR) with IC50 of 9.85 μM, 7.7 μM, and 6.18 μM respectively. In vitro, this multi-kinase inhibitor causes apoptosis in HT-29 colon cancer cells, and in a C. elegans worm study, showed a weak but significant increase in lifespan. A six week double-blind, placebo-controlled study involving 73 healthy volunteers demonstrated that the MP mixture is safe to consume orally. All clinical blood markers remained within normal levels and no clinically significant side effects were reported. There was some evidence of improved insulin sensitivity, neutrophil-to-lymphocyte ratio (NLR), and quality of life assessment of role of physical function.


These data in combination with the observed in vitro anti-cancer properties warrant further clinical studies to investigate this MP mixture as a potential clinical nutrition intervention for improving the quality of life and clinical outcomes in cancer patients.

Trial Registration


Health; Cancer; Neutrophil Lymphocyte Ratio; Insulin Sensitivity; Quality of Life