Table 1 |
||
|
The primers used in the Real Time-PCR. |
||
|
Genes |
GenBank accession number |
Primers |
|
|
||
|
MAT |
F: 5'- ACTTTGTTCCCGGGAGCTGTC -3' |
|
|
R: 5'- AACTGCATGCCAATTATTCTGCTG -3' |
||
|
SAHH |
F: 5'- CACCACAGGCTGTATTGACATCATC -3' |
|
|
R: 5'- GTCCAATGTTACACACAATGGCATC -3' |
||
|
MTR |
F: 5'-TAAGATTTGCAAAGGTTGGGTCTGA-3' |
|
|
R: 5'-CTGGACATACAGGTGGGAGTTGG-3' |
||
|
MTHFR |
F: 5'- TGTGTGAATTCTGCAACTAGCCAAG -3' |
|
|
R: 5'- ATGAGCCACCACACCTGCTG -3' |
||
|
CSE |
F: 5'-CCTTTGGCTCTGGGAGCTGATA-3' |
|
|
R: 5'-ATTAACAGACACCAGGCCCATTACA-3' |
||
|
CBS |
F: 5'-TGTGGGCACACCATCGAGA-3' |
|
|
R: 5'-AGCGTCACCATTCCCAGGATTA-3' |
||
|
β-actin |
F: 5'- CGACAACGGCTCCGGCATGT-3' |
|
|
R: 5'- TGGGCCTCGTCGCCCACATA-3' |
||
|
|
||
|
MAT: methionine adenosyltransferase; SAHH: S-adenosylhomocysteine hydrolase; MTR: 5-methyltetrahydrofolate-homocysteine methyltransferase; MTHFR: 5, 10-methylenetetrahydrofolate reductase; CSE: cystathionine γ-lyase; CBS: cystathionine β-synthase; β-action: housekeeping gene |
||
|
Huang et al. Nutrition Journal 2012 11:6 doi:10.1186/1475-2891-11-6 |
||
