Table 6

Serum concentrations of amino acids at baseline and changes after 4 wk and 8 wk of intervention with LPI and MPI
LPI MPI
Changes from baseline Changes from baseline Pa Pa
Amino acid (μmol/L) Baseline wk 4 wk 8 wk 4 wk 8 wk 4 wk 8
Alanine 425.0 ± 147.6 −1.1 ± 98.2 1.8 ± 132.2 45.1 ± 102.0* 2.6 ± 121.8 0.022 0.96
Arginine 96.2 ± 30.3 1.0 ± 24.7 9.0 ± 28.4 4.6 ± 28.3 3.1 ± 29.8 0.43 0.18
Asparagine 48.9 ± 13.9 2.8 ± 16.0 7.2 ± 16.3* 7.8 ± 12.5*** 3.1 ± 12.1 0.08 0.09
Aspartateb 19.6 ± 8.9 −4.0 ± 10.5* −6.2 ± 7.5*** −1.1 ± 8.5 −6.8 ± 8.9*** 0.22 0.56
Cystine 65.2 ± 18.5 −3.3 ± 12.5 1.9 ± 16.0 2.2 ± 12.4 1.4 ± 15.9 0.025 0.81
Glutamine 623.7 ± 169.6 −22.7 ± 105.8 14.2 ± 132.0 30.6 ± 132.1 19.0 ± 150.4 0.026 0.84
Glutamateb 55.5 ± 27.8 −9.3 ± 25.2* −14.6 ± 20.3** 3.3 ± 28.9 −15.1 ± 22.0** 0.038 0.89
Glycine 246.1 ± 82.4 −14.5 ± 50.5 −10.6 ± 63.8 0.8 ± 58.1 −13.4 ± 66.8 0.17 0.76
Histidine 90.5 ± 26.1 −4.9 ± 19.0 6.9 ± 21.5 8.2 ± 21.6 3.5 ± 23.3 0.001 0.24
Isoleucine 63.5 ± 32.0 3.2 ± 28.2 4.2 ± 27.6 13.4 ± 31.3* 2.9 ± 28.3 0.014 0.70
Leucine 145.2 ± 48.4 0.9 ± 40.9 7.0 ± 39.6 24.5 ± 48.1** 6.4 ± 41.8 0.002 0.91
Lysine 200.2 ± 57.2 −8.4 ± 50.7 9.8 ± 53.3 29.7 ± 58.0** 18.0 ± 58.4 0.0001 0.29
Methionine 22.9 ± 7.8 −1.9 ± 7.1 0.4 ± 7.4 3.8 ± 8.3* 0.5 ± 7.8 < 0.0001 0.96
Phenylalanine 66.8 ± 16.4 −1.4 ± 15.8 1.7 ± 18.5 8.4 ± 18.0* 1.6 ± 18.4 0.003 0.98
Prolineb 169.7 ± 99.9 18.8 ± 112.9 24.5 ± 101.0 81.8 ± 184.1** 39.7 ± 117.2 0.007 0.31
Serineb 112.4 ± 35.6 −9.8 ± 29.2 −3.8 ± 30.8 5.1 ± 28.9 −4.6 ± 27.0 0.042 0.87
Threonineb 132.5 ± 49.7 −6.0 ± 35.8 2.2 ± 39.7 14.6 ± 37.1 7.7 ± 40.8 0.013 0.38
Tryptophan 76.0 ± 27.7 −7.3 ± 25.2 9.4 ± 44.9 4.8 ± 28.6 −1.3 ± 28.2 0.010 0.13
Tyrosine 75.2 ± 22.8 −0.5 ± 18.8 4.2 ± 21.3 16.0 ± 27.7** 4.3 ± 19.0 0.002 0.98
Valine 243.5 ± 87.3 −3.8 ± 75.8 13.4 ± 64.5 45.6 ± 78.1** 24.6 ± 79.2 0.0004 0.29
EAA 1041.1 ± 313.0 −29.5 ± 261.3 54.9 ± 254.4 153.2 ± 302.9** 63.8 ± 297.5 0.0003 0.79
Non-EAA 1937.4 ± 507.4 −42.3 ± 382.2 27.7 ± 458.8 196.2 ± 478.1* 33.2 ± 458.2 0.005 0.93
BCAA 452.2 ± 165.4 0.3 ± 142.1 24.6 ± 126.8 83.6 ± 154.7** 33.9 ± 145.3 0.001 0.62
Lysine:arginine ratio 2.16 ± 0.55 −0.14 ± 0.32* −0.04 ± 0.28 0.17 ± 0.35** 0.10 ± 0.34 0.0004 0.16

Values are presented as means ± standard deviation, n = 33.

Abbreviation: LPI lupin protein isolate, MPI milk protein isolate, EAA essential amino acids, Non-EAA non-essential essential amino acids, BCAA branched chained amino acids.

aP-value is for differences between treatments at wk 4 or at wk 8 determined by repeated measures ANOVA.

b Data were not normally distributed and/or had heterogeneous variances, and thus were statistically analyzed using a linear mixed model.

*, **, *** Significant differences comparing wk 4 and wk 8 with baseline determined by repeated measures ANOVA (*P ≤ 0.050, **P ≤ 0.010, ***P ≤ 0.001).

Bähr et al.

Bähr et al. Nutrition Journal 2013 12:107   doi:10.1186/1475-2891-12-107

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