Table 1

Composition of experimental diets
Ingredient Standard bean diet Biofortified bean diet
g/kg (by formulation)
Biofortified Beans (88 μg Fe/g) _ 400
Standard Beans (59 μg Fe/g) 400 _
Corn 350 350
Corn oil 30 30
Dry skim milk 100 100
Corn starch 46.75 46.75
Vitamin/mineral premix (no Fe) 1 70 70
DL-Methionine 2.5 2.5
Choline Chloride 0.75 0.75
Total (g) 1000 1000
Selected components mean ± SEM, n = 5 (by analysis)
Fe (μg Fe/g) 2 39.4 ± 0.2b 52.9 ± 0.9a
Phytate:Fe molar ratio3 8.25 ± 0. 65a 8.95 ± 0.72a

1Vitamin and mineral premix provided/kg diet (330002 Chick vitamin mixture; 235001 Salt mix for chick diet; Dyets Inc. Bethlehem, PA).

2Iron concentrations in the diets were determined by an inductively-coupled argon-plasma/atomic emission spectrophotometer (ICAP 61E Thermal Jarrell Ash Trace Analyzer, Jarrell Ash Co. Franklin, MA) following wet ashing.

3Method for determining phytate contents are described in the materials and methods section.

a,bWithin a row, means without a common letter are different, P < 0.05.

Tako et al.

Tako et al. Nutrition Journal 2014 13:28   doi:10.1186/1475-2891-13-28

Open Data