Table 5

Origin, enzymatic pathways of reactive oxygen species, and their oxidized products.

Origin – Location Enzymatic Pathway
ROS Potent Oxidants
PRODUCTS Oxidized lipids and Proteins

Mitochondrial Respiratory Chain
O2-OH
Oxidized lipids, proteins, nucleic acids, and autoxidation byproducts.
Inflammatory Macrophage Membraneous NAD(P)H Oxidase
O2OHH2O2
Advanced Lipoxidation Endproducts (ALE) Ortho o-tyrosine Meta m- tyrosine
Granular Myeloperoxidase (MPO)
Hypochlorous Acid HOCL (bleach) Tyr NO2
3-Chlorotyrosine di-Tyrosine NO2-Tyrosine (Nitrotyrosine)
Macrophage Nitric Oxide Synthase (NOS) Inducible (iNOS) Large bursts uncontrolled
ONOO'
NO2-Tyr (Nitrotyrosine)
Endothelial Cell


Nitric Oxide Synthase (NOS) Constitutive (cNOS) eNOS → NO nNOS → NO Small bursts (puffs) controlled
NO + O2→ ONOO' ONOO'
NO2-Tyr (Nitrotyrosine) NO2-Tyr
eNOS derived NO
NO: the GOOD
Natural Occurring, Local Occurring, Chain Breaking Antioxidant
Superoxide
O2.: the BAD

Peroxynitrite
ONOO : the UGLY

Hypochlorous acid
HCLO : the UGLY

Restoration of endothelial derived NITRIC OXIDE Via the eNOS reaction..
ANTIOXIDANT ANTIREDOXIDANT → → →
Prevention of the devastating effects of ROS.

Hayden and Tyagi Nutrition Journal 2004 3:4   doi:10.1186/1475-2891-3-4