Apoptosis induction analyzed by TUNEL assay in cervical cells. Cells were treated with 5% and 25% cactus pear solution for 2 days. Cells were harvested and incubated with TdT in the presence of biotin-labeled BrdU and analyzed by flow cytometry. The percentage of apoptotic cells is represented by dark dots (fluorescence of individual cells) above the line in R3 region (R3 is the computer software analysis apoptosis program).
Zou et al. Nutrition Journal 2005 4:25 doi:10.1186/1475-2891-4-25