|
Micronutrient assay methods. |
||
| Analyte |
Method |
Ref. |
|
|
||
| RBC folate (nmol/L) |
Quantaphase Folate Radioassay Kit, Bio-Rad Laboratories |
7 |
| Retinol (μg/dL) |
Isocratic reversed-phase HPLC with UV/Vis detection |
8 |
| Ascorbic acid (mg/dL) |
Isocratic reversed-phase HPLC with Electrochemical detection* |
7 |
| Alpha tocopherol (mg/dL) |
Isocratic reversed-phase HPLC with UV/Vis detection |
8 |
| Alpha carotene (μg/dL) |
Isocratic reversed-phase HPLC with UV/Vis detection |
8 |
| Beta carotene (μg/dL) |
Isocratic reversed-phase HPLC with UV/Vis detection |
8 |
| Ferritin (μg/L) |
Quantlmune Ferritin IRMA kit, Bio-Rad Laboratories |
7 |
| 25-hydroxyvitamin D (nmol/L) |
Quantlmune Ferritin IRMA kit, Bio-Rad Laboratories |
7 |
| Homocysteine (μmol/L) |
Isocratic reversed-phase HPLC with Fluorescence detection |
9 |
| C-reactive protein (mg/L) |
Latex-enhanced nephelometry, Behring Nephelometer Analyzer for NHANES assays and Hitachi 917 Analyzer for Multiple Supp user assays |
7 |
| Total cholesterol (mg/dL) |
Timed endpoint, coupled enzymatic methodology |
10 |
| LDL-cholesterol (mg/dL) |
Calculated** |
7 |
| HDL-cholesterol (mg/dL) |
Polyanion/divalent cation precipitation of other lipoproteins followed by timed endpoint, coupled enzymatic methodology |
10 |
| Triglycerides (mg/dL) |
Timed endpoint, coupled enzymatic methodology |
11 |
|
*Use of 1 part serum plus 1 part 10% meta-phosphoric acid for Multiple Supp users versus NHANES use of 1 part serum plus 4 part meta-phosphoric acid. ** Calculated: LDL-cholesterol = [total cholesterol - HDL-cholesterol - (triglycerides/5)] Ref = reference, LDL = low-density lipoprotein, HDL = high-density lipoprotein. | ||
Block et al. Nutrition Journal 2007 6:30 doi:10.1186/1475-2891-6-30 |
||