Table 2

The mobile phases studied.

No

Solvents

Proportions (v/v)

1

toluene – ethyl acetate – formic acid (98–100%)

36 : 12 : 5

2

cyclohexane – ethyl acetate – formic acid (98–100%)

30 : 15 : 5

3

toluene – ethyl acetate – glacial acetic acid

36 : 12 : 5

4

cyclohexane – ethyl acetate – glacial acetic acid

31 : 14 : 5

5

n-hexane – ethyl acetate – formic acid (98–100%)

31 : 14 : 5

6

toluene – acetone – formic acid (98–100%)

38 : 10 : 5

7

n-hexane-ethyl acetate – glacial acetic acid

31 : 14 : 5

8

petroleum ether (40–70°C) – ethyl acetate – formic acid (98–100%)

30 : 15 : 5

9

carbon tetrachloride – acetone – formic acid (98–100%)

35 : 10 : 5

10

n-hexane – ethyl acetate – glacial acetic acid

30 : 20 : 1.5

11

chloroform – methanol – formic acid (98–100%)

44 : 3.5 : 2. 5

Medić-Šarić et al. Nutrition Journal 2009 8:33   doi:10.1186/1475-2891-8-33