Leptin mRNA and protein levels. Panel A: Serum leptin levels. Panel B: Adipose tissue leptin mRNA levels measured by StellARray. Panel C: Adipose tissue leptin mRNA levels measured by qRT-PCR. The data in this Fig is the relative mRNA change in selected genes in the adipose tissue of CR, LF, and HF mice consuming water or 20% alcohol relative to LF mice consuming water. If the ratio is higher than 1, the mRNA content for that gene was amplified; if the ratio is below 1, the mRNA content decreased. The ratio for the LF mice consuming water is 1 because the mRNA level for that gene was the control group.
Paulson et al. Nutrition Journal 2010 9:14 doi:10.1186/1475-2891-9-14