Figure 1.

Specificity of the vitamin B1 and B6 assay. Chromatograms of PN placebo (A and D), spiked with the B1 and B6 (B and E) and PN containing the multivitamin (C and F) obtained by HPLC at wavelength of 250 and 295 nm, respectively, using column of reverse phase C18 and a mixture of methanol:water (27:73, v/v) and sodium hexano sulfonate 1,4%, pH 3.0 as mobile phase (n = 3).

Ribeiro et al. Nutrition Journal 2011 10:47   doi:10.1186/1475-2891-10-47
Download authors' original image