Cell death analysis of splenic lymphocytes by trypan blue assay. Splenic lymphocytes were cultured in the presence of H2O2, MRN-100, MRN-100 + H2O2, or control (CM). At 16 hrs, cells from the different groups were treated with trypan blue stain for 5 minutes and counted. The percent of dead cells was calculated out of a total of 300 cells. * indicates p < 0.02 as compared to cells treated with H2O2. Data shown is representative of three independent experiments.
Ghoneum et al. Nutrition Journal 2009 8:18 doi:10.1186/1475-2891-8-18