Average DNA yield obtained using the four commercial kits as influenced by fecal dry matter. The percent dry matter in the human fecal specimens were 26%, 35%, and 41% (n = 45/kit). Values for DNA yield were normalized based on the dry weight of the respective fecal sample. DNA from the fecal specimens were extracted using Mobio Ultra Clean® Fecal DNA Isolation Kit (M), QIAamp® DNA Stool Mini Kit (Q), FastDNA® SPIN Kit (FSp), and FastDNA® SPIN Kit for Soil (FSo).
Ariefdjohan et al. Nutrition Journal 2010 9:23 doi:10.1186/1475-2891-9-23