Table 2 |
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Average DNA yield obtained using the four commercial DNA extraction kits1. |
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DNA extraction kit1 |
DNA yield (mg DNA/g dry wt feces)3 |
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M |
52.4 ± 14.5b |
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Q |
57.0 ± 22.6b |
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FSp |
151.3 ± 47.1a |
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FSo |
187.2 ± 69.4a |
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The following extractions accounted for various fecal specimen weights (10, 25, 50, 100, and 200 mg) and DNA yield was normalized by percent fecal dry matter (26%, 35%, and 41%)2. 1M, Mobio Ultra Clean® Fecal DNA Isolation Kit; Q, QIAamp® DNA Stool Mini Kit; FSp, FastDNA® SPIN Kit; FSo, FastDNA® SPIN Kit for Soil 2Values of DNA yield were based on n = 45/DNA extraction method and were normalized based on the dry weight of the respective fecal sample 3Treatment groups with different letters indicate significant differences between groups (P < 0.05). Values are means ± SE. |
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Ariefdjohan et al. Nutrition Journal 2010 9:23 doi:10.1186/1475-2891-9-23 |
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